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2021-06-03
Military Medical Centre of the Hungarian Defence Forces, H-1134, Budapest, Róbert Károly krt. 44, Hungary; Semmelweis University - Faculty of Medicine, H-1085, Budapest, Üllői út 26., Hungary.; Military Medical Centre of the Hungarian Defence Forces, H-1134, Budapest, Róbert Károly krt. 44, Hungary; Semmelweis University - Faculty of Medicine, H-1085, Budapest, Üllői út 26., Hungary.; Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungária krt. 21, H-1143, Budapest, Hungary.; Military Medical Centre of the Hungarian Defence Forces, H-1134, Budapest, Róbert Károly krt. 44, Hungary.; Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary.; Military Medical Centre of the Hungarian Defence Forces, H-1134, Budapest, Róbert Károly krt. 44, Hungary.; Barts Health NHS Trust, The Royal London Hospital, 80 Newak Street, London, United Kingdom.; Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary.; Military Medical Centre of the Hungarian Defence Forces, H-1134, Budapest, Róbert Károly krt. 44, Hungary; Semmelweis University - Faculty of Medicine, H-1085, Budapest, Üllői út 26., Hungary. Electronic address: lgvirology@gmail.com.
Seeing the global emergence and the lack of a definitive cure for COVID-19, it is essential to find the most sensitive and specific detection method to identify infected patients in a timely manner. Our paper aims to compare the clinical sensitivity of different commercial RT-qPCR (Genesig, 1copy, DNA-Techonolgy and Charité primer-probe sets), isothermal PCR (Ustar Isothermal Amplification-Real Time Fluorescent Assay) and immunochromatographic antigen detection (BIOCREDIT COVID-19 Ag) assays developed to use in laboratory diagnosis of COVID-19. A total of 119 nasopharyngeal swab specimens were collected from symptomatic patients. A subset of samples, positive with two RT-qPCR assays were then tested with isothermal PCR and rapid antigen tests. Of the 119 specimens, 65 were positive by at least two PCR assays. All PCR assays showed substantial or perfect match, although some variations in the clinical performance was observed. Of the 37 and 32 remnant nasopharyngeal samples positive by